Journal: Journal of Inflammation Research
Article Title: Vitamin D Binding Protein: A Potential Factor in Geriatric COVID-19 Acute Lung Injury
doi: 10.2147/JIR.S470097
Figure Lengend Snippet: The VDBP expression in injured alveolar epithelial cells. ( A ) The VDBP protein level in LPS-induced injury of alveolar epithelial cells at different time points. The representative Western blot results and the relative expression of VDBP from the Western blot were shown. ( B ) The VDBP gene expression in the LPS-induced injury of alveolar epithelial cells. The q-PCR was used to analyze the relative expression of genes. Columns and error bars represented mean ± SEM. ** P < 0.01, *** P < 0.001. VDBP: Vitamin D-binding protein, LPS: Lipopolysaccharide, NC: negative control, GAPDH: glyceraldehyde-3-phosphate dehydrogenase.
Article Snippet: Then separate equal amounts of proteins on 12% SDS-PAGE for Western blot using antibodies against VDBP (1:2000 dilution, Proteintech, US) and GAPDH (1:5000 dilution, Proteintech, US), the protein was observed using the ECL chemiluminescence method.
Techniques: Expressing, Western Blot, Gene Expression, Binding Assay, Negative Control